LOCUS       40924_22819        3792 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGV4112 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Broeders S, De Greve H, De Rycke R, Bauw G, Jansens S, Buys L, 
            Smagghe G, Ripoll C, Peumans WJ, Van Damme EJM., Hernalsteens JP.
  TITLE     Leaf and bulb lectins from garlic (Allium sativum L.) are expressed,
            correctly processed and targeted to the cytoplasm in transgenic 
            tobacco plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   De Greve H.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2001) De Greve H., Genetische Virologie, Vrije 
            Universiteit Brussel, Paardenstraat 65, Sint-Genesius-Rode, B-1640, 
            BELGIUM
REFERENCE   3  (bases 1 to 3792)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2001) De Greve H., Genetische Virologie, Vrije Universiteit 
            Brussel, Paardenstraat 65, Sint-Genesius-Rode, B-1640, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        910..1255
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    1264..1290
                     /label=multiple cloning site
                     /note="multiple cloning site"
     terminator      1301..1553
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1571..1587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1595..1611)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1619..1649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1664..1685)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1973..2561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2735..3592)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3593..3697)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcc
      421 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
      481 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
      541 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
      601 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
      661 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
      721 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
      781 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
      841 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
      901 aagggcaatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      961 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     1021 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     1081 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     1141 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1201 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     1261 gactctagag gatccccggg taccgagctc gaatttcccc gatcgttcaa acatttggca
     1321 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     1381 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     1441 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     1501 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     1561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2161 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2401 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     3301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     3361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     3421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3721 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3781 ggccctttcg tc
//
